Human Pluripotent Stem Cell Core

Overview of Services  

The Human Pluripotent Stem Cell (HPSC) Core, established in 2008, is part of the Raymond G. Perelman Center for Cellular and Molecular Therapeutics.  An infrastructure and solid foundation has been created for the generation, differentiation, and gene editing of induced pluripotent stem cell (iPSC) lines. Using the CRISPR-CAS9 gene editing technology, isogenic control and disease-specific iPSC lines can be created for modeling human disease to study mechanism, development, and establish new therapeutic modalities. The Core provides enrichment training courses for 1) growth and maintenance of iPSC lines, 2) differentiation to mesoderm, endoderm or ectoderm, and 3) gene editing.

GETTING ACCESS TO OUR FACILITY

Researchers who would like to start purchasing from our core must have an iLab account and a form of payment set in iLab.

  - Sign-up for an iLab account at https://chop.ilab.agilent.com/landing/101

  - The steps for setting up a form of payment in iLab depend on your affiliation: CHOP, UPenn or another institution. 

• Researchers from CHOP who would like to start using our facility should take the following steps:
• Ask your PI or business manager to give you access in iLab to an active account.
• Place orders using an active funding source from the drop down menu.

• Researchers from Penn who would like to purchase from our core should take the following steps:
• Make sure you have an active iLab account. If necessary, sign up for an iLab account. 
• Request a purchase order (PO) from your business manager for covering the purchases from our core. 
• In order to generate the PO, your business manager may ask for our BenBuys information, which is Phila 200, Site 49513, Children's Hospital of Philadelphia.
• Be aware that the PO does not trigger any payment, it only sets the upper limit for the dollar amount that we are allowed to invoice.
• At the beginning of each month, our administrative team will send invoices for the services performed to Penn’s central Accounts Payable.
• You can check the balance on your existing POs in iLab, by clicking on the PO tab.
• The end-user is responsible for making sure that the correct PO is being used for purchases. Consult with your business manager if you have any doubts.
• Enter the PO number in iLab. All end users and lab managers should be able to enter a PO in iLab. The PO will be reviewed and approved within two business days by our administrative team. 
• You may proceed with ordering as soon as the PO is available.   
• Request a new PO and enter it in iLab when the initial funds are running low. You will not be able to purchase in iLab if your funding is insufficient.
• For adding new lab members to an existing PO  please send an email message to CHOP Core Facilities Administration at CoresAdmin@email.chop.edu. Please note that the Core staff members cannot make any changes to existing POs.
• For general questions about iLab or about setting up a new PO, Penn researchers should contact April Weakley at iLab.Support@Pennmedicine.upenn.edu
  
  
• IMPORTANT: We request all Penn users apply for a CHOP token as soon as possible.  The token is a small device affixed  to the Penn ID, which allows for easy access into our building between 8AM and 6PM, without checking in with the security officer in the lobby. Without a CHOP token, access to our lab is possible only when the staff is present. A photo ID will be required by security and the access to the lab may be delayed.

Please contact the CHOP Core Facilities Administration at CoresAdmin@email.chop.edu if you have inquiries about setting up a form of payment or about your iLab account.

Location and hours of operation

Contacts