Flow Cytometry Core Laboratory

 

Overview of Services

The Flow Cytometry Core Laboratory provides access to state of the art instrumentation and professional flow cytometry services to members of the research community of The Children's Hospital of Philadelphia and University of Pennsylvania. The lab is located in Suite 1207 on the 12th floor of the Leonard and Madlyn Abramson Pediatric Research Center. Our staff has extensive expertise in performing a variety of flow cytometry applications, including sample processing for surface and intracellular staining, functional assays, complex multi-color flow analyses and cell sorting. Most cytometers and sorters are located in Suite 1207 in Abramson Research Center (ARC) at CHOP.  

 

INSTRUMENTS

 

The instruments listed below are available in the Flow Cytometry Core Lab at CHOP. We also offer access to software for flow data analysis.

Instruments

   Analyzers:
  • Aurora A (ARC 1207i;  5 lasers, 64 detectors)
  • Aurora B (CTRB 10107;  5 lasers, 64 detectors)
  • CytoFLEX LX (6 lasers, 21 colors)
  • CytoFLEX S (ARC1207;  4 lasers, 13 colors)
  • CytoFLEX S (ARC1207;  3 lasers, 13 colors)
  • LSRFortessa (ARC1207;  5 lasers, 18 colors)
  • LSR II (CTRB 10107;  4 lasers, 15 colors)
   Sorters:
   Imaging system:
   Cell counter
  Software for flow cytometry data analysis
  • FlowJo and FCS Express (annual licenses can be ordered by logging in and clicking the Request Services tab)

FCS Express includes the Image cytometry, High content analysis  and Advanced multi-cycle analysis.

For an introduction to FCS Express, watch the recording of the webinar we hosted on September 21, 2022.

Download the Optical Configuration of our instruments.

 

SERVICES

  • Access to analyzers and software
  • Cell sorting
  • Hands-on training on cytometers and sorters
  • Sample preparation for surface and intracellular staining
  • Polychromatic flow cytometry assays for clinical research studies
  • Functional assays (intracellular calcium, apoptosis, cell cycle etc.)

Full-service sorting and semi-assisted sorting are available during business hours. Unassisted sorting is available during evenings and weekends.

 * Download the Guidelines for Planning a Cell sorting Experiment in our facility.
 * Download the Fee Schedule.

 

TRAINING

  • For training sessions on analyzers please log in iLab, click the Request Services tab and submit  a Training request
  • One-on-one training sessions for cell sorting should be scheduled directly on the iLab calendar of each sorter

For a review of basic flow cytometry concepts,check out the Molecular Probes tutorials prior to scheduling any training session on an instrument: 

Other flow cytometry resources:

  • The Art of Sorting: a detailed presentation of the principles of electrostatic droplet sorting. 

 

ACCESSIBILITY

  • Researchers from CHOP who need to start using our faciliaty should take the following steps:
    • Register for an iLab account at https://chop.ilab.agilent.com/landing/101 
    • Ask your PI or business manager to give you access in iLab to an active account.  
    • You may proceed with reserving cytometers, scheduling sorting or training on the iLab calendars, which can be accessed by scrolling all the way down to the bottom of this page.
    • Our lab is open 24/7 to researchers who carry a CHOP badge. The staff is present during business hours (9 am to 5 pm during working days).
  • Researchers from Penn who would like to use our facility should take the following steps:
    • Make sure you have an active iLab account. If necessary, sign up for an iLab account
    • Request a purchase order (PO) from your business manager for covering the utilization fees in our lab. 
      • In order to generate the PO, your business manager may ask for our BenBuys information, which is Phila 200, Site 49513, Children's Hospital of Philadelphia.
      • Be aware that the PO does not trigger any payment, it only sets the upper limit for the dollar amount that we are allowed to invoice.
      • At the beginning of each month, our administrative team will send invoices for the services performed to Penn’s central Accounts Payable.
      • You can check the balance on your existing POs in iLab, by clicking on the PO tab.
      • The end-user is responsable for making sure that the correct PO is being used for each reservation on our calendars. Consult with your business manager if you have any doubts.
      • Since iLab does not allow end-users to edit past reservations, you must inform us immediately by emailing to flowcytometry@chop.edu if you notice that a charge for a reservation should have been set on a different PO.
        The Flow Core staff will make the necessary change on your behalf.
    • Enter the PO number in iLab. All end users and lab managers should be able to do this task. The PO will be reviewed and approved within two business days by our administrative staff. 
      • You may proceed with reserving equipment in iLab as soon as the PO is available.   
      • Request a new PO and enter it in iLab when the initial funds are running low. You will not be able to set up reservations in iLab if your funding is insufficient.
    • Please note: We request all Penn users apply for a CHOP token as soon as possible.  The token is a small device affixed  to the Penn ID, which allows for easy access into our building between 8AM and 6PM, without checking in with the security officer in the lobby. Without a CHOP token, access to our lab is possible only when the staff is present. Photo ID will be required by security and the access to the lab may be delayed.
  • Researchers from other institutions are required to have a formal research agreement with our facility. Unassisted or semi-assisted access to our instruments is not available for researcher from outside our campus.

Please email to CoresAdmin@email.chop.edu if you have questions about your iLab account or setting up a form of payment.

 

BIOSAFETY

Our lab is organized as a Biosafety Level 2 (BSL-2) facility. Whenever possible, samples brought to the lab should be fixed using a method that effectively inactivates pathogens (e.g. formaldehyde, paraformaldehyde, detergents, high temperature).

A biosafety questionnaire is required for each reservation. The staff will assess the biosafety risks for your samples. We thank you in advance for your cooperation with keeping everyone safe.

The following requirements should be met by researchers present in the flow lab:

    • Gloves should be used at all times while touching equipment or accessories in the Flow lab.
    • lab coat is required when working in the flow Lab. We have a limited number of clean lab coats, please plan on bringing your own.
    • Samples should be filtered immediately prior to loading them on sorters to minimize the risks of clogging
      We have posted by each sorter checklists on how to safely handle a clogged sorter.
    • Plan your flow or sorting experiment ahead of time; we will need time to review and address any biosafety issues.

We welcome your suggestions for maintaining the highest level of safety in the Flow Lab!

 

Leadership and Staff

Florin Tuluc, MD, PhD
Director

Jennifer Murray 
Senior technical specialist

John Lora 
Technical specialist

Angela Mckenzie
Technical specialist

Kevin Liedel
Technical specialist

Chloe Davis
Technical specialist

Sophia McKee
Technical specialist

Jorgi Strother
Technical Specialist

 

Links and Resources

  1. Flow Core Lab -- CHOP website
  2. Planning your first cell sorting experiment
  3. The optical configuration of our instruments
  4. Other core labs at CHOP

Location and hours of operation

Hours of operation:   Open: 24/7      |    Staffed: Monday - Friday 9 AM to 5 PM

Location:  3615 Civic Center Boulevard, Abramson Research Building, room 1207, Philadelphia, PA 19104 (see the map below)

General questions or inquiries should be sent by email at flowcytometry@chop.edu and they will be auto-forwarded to all members of the flow lab.

Phone: 215-590-3402 

 

Available Equipment and Resources

Analyzers (8)
Other (3)
Sorters (6)

Map