Flow Cytometry Core Laboratory

Astrios Nozzle

 

Overview of Services

The Flow Cytometry Core Laboratory provides access to state of the art instrumentation and professional flow cytometry services to members of the research community of The Children's Hospital of Philadelphia and University of Pennsylvania; investigators from outside the campus are welcome to our facility. The lab is located in Suite 1207 on the 12th floor of the Leonard and Madlyn Abramson Pediatric Research Center. The staff has the expertise in performing a variety of flow cytometry applications, including sample processing for surface and intracellular staining, functional assays, complex multi-color flow analyses and cell sorting. 
Most cytometers and sorters are located in Suite 1207 in Abramson Research Center (ARC) at CHOP, with the exception of Aurora B (located in CTRB 10107), FACSCalibur (located in ARC 313) and CytoFLEX S (4 lasers; located in ARC 1214C).  

  

  • The Flow Lab is open 24/7 to users with a CHOP badge. Researchers from other institutions have access to the building only during business hours. 

  • Researchers from Penn who would like to use our facility should take the following steps:
    • Make sure you have an active iLab account. If necessary, sign up for an iLab account
    • Request a purchase order (PO) from your business manager for covering the utilization fees in our lab. 
      In order to generate the PO, your business manager may ask for our BenBuys information, which is Phila 200, Site 49513, Children's Hospital of Philadelphia.
    • Enter the PO number in iLab. All end users and lab managers should be able to do this task. The PO will be reviewed and approved within two business days by our administrative staff. 
    • You may proceed with reserving equipment in iLab as soon as the PO is entered in iLab. Email to flowcytometry@chop.edu or call at 215-590-3402 if you have any questions.  
    • Request a new PO and enter it in iLab when the initial funds are running low. You will not be able to set up reservations in iLab if your funding is insufficient.

    • Email to CoresAdmin@email.chop.edu if you have general questions about iLab access. 

    • Optional: Apply for a CHOP token that gives you easy access to our building without checking in with security. The token will also unlock the entrance in our main lab in ARC 1207. This step is not absolutely necessary if you will come to our lab only during business hours, but it is highly recommended. Without a token you may not be able to enter the lab if no staff is present. 

 

BIOSAFETY

Our lab is organized as a Biosafety Level 2 (BSL-2) facility. Whenever possible, samples brought to the lab shuld be fixed using a method that effecttively inactivates pathogens (e.g. formaldehyde, paraformaldehyde, detergents, high temperature).

A biosafety questionnaire is required for each reservation. The staff will assess the biosafety risks for your samples and we thank you in advance for your patience for your cooperation in keeping everyone safe.

The following requirements should be met by researchers present in the flow lab:

  • Gloves should be used at all times while touching equipment or accessories in the Flow lab.
  • A face mask is mandatory at this time.
  • lab coat is required when working in the flow Lab. We have a limited number of clean lab coats, please plan on bringing your own.
  • We ask for your cooperation in order to maintain social distancing.
  • The work place must be wiped with disinfectant before and after the completion of the work.
  • Samples should be filtered immediately prior to loading them on sorters to minimize the risks of clogging. We have posted by each sorter checklists on how to safely handle a clogged sorter.
  • Plan your flow or sorting experiment ahead of time; we will need time to review and address any biosafety issues.

 

 

TRAINING

One-on-one training session on instruments may be scheduled by sending an email to flowcytometry@chop.edu.

For a review of basic flow cytometry concepts check out the Molecular Probes tutorial series: 

 

SERVICES

 

Full-service sorting and semi-assisted sorting are available during business hours. Unassisted sorting is available during evening and weekends.

 

We welcome your suggestions for improving the workflow and maintain the highest level of safety in the Flow Lab!

 

General questions or inquiries should be sent by email at flowcytometry@chop.edu and they will be auto-forwarded to all members of the flow lab. 

 

 

Instruments

Instruments

Click on the links below to check the availability of each instrument without signing in. 
To reserve instruments you must Sign in.

   Analyzers:
   Sorters:

 

   Imaging system:
 Cell counter
Software for flow cytometry data analysis
  • FlowJo (licenses are available for $275/year) 
  • FCS Express (shared licenses using internet dongles, free of charge)

 



Services

  • Access to analyzers and software
  • Cell sorting
  • Hands-on training on cytometers and sorters
  • Sample preparation for surface and intracellular staining
  • Polychromatic flow cytometry assays for clinical research studies
  • Functional assays (intracellular calcium, apoptosis, cell cycle etc.)

Accessibility

  • The lab is open 24/7 to researchers who carry a CHOP ID.
  • Users from other institutions have access during business ours and they will need to sign in with the security guard in the lobby.
  • Easy Access to CHOP Core Facilities for Frequent Penn Users is available using a token affixed to the Penn ID.
  • Penn BenBuys Information for for Penn researchers: Phila 200, Site 49513.

Leadership and Staff

Florin Tuluc, MD, PhD
Director

Jennifer Murray 
Senior technical specialist

John Lora 
Technical specialist

Amie Albertus
Technical specialist

Angela Mckenzie
Technical specialist

Kevin Liedel
Technical specialist

 

Links and Resources

  1. Flow Cytometry Core Laboratory (main website)
  2. FAQs
  3. Getting started
  4. Planning your first cell sorting experiment
  5. The optical configuration of our instruments
  6. Other core labs at CHOP

Location and hours of operation

Hours of operation:   Open: 24/7      |    Staffed: Monday - Friday 9 AM to 5 PM

Location:  3615 Civic Center Boulevard, Abramson Research Building, room 1207, Philadelphia, PA 19104 (see the map below)

Available Equipment and Resources

Analyzers (9)
Other (3)
Sorters (5)

Map