CHOP High Throughput Sequencing Core

Overview of Services

HT Sequencing Core is a full-service NGS facility providing instrumentation, library preparation, and sequencing services to CHOP, UPenn, and to some extent to external investigators. Whether you want to sequence libraries that you generated yourself, or assistance with library preparation, we can help. Our goal is to enable in-house high throughput sequencing service and to make the HTS Core accessible to everyone interested in sequencing projects. 

HT Sequencing Core has capabilities ranging from highly focused analysis of miRNA to whole genome, epigenome, exome, and transcriptome sequencing. Services are available for a diversity of study designs; starting with as few as 12 samples to large projects involving hundreds of samples, we are working with investigators to provide optimized protocols for each study. We understand that not all research is alike, we offer consulting services for custom experiments tailored to your research needs. We are here to listen and create solutions.

For any sequencing project, except self-servce, please request a project ID. Samples can be submitted only after the requested service has been aproved in iLab and a project ID has been issued. For sample drop off please contact us through email, sample dropoff M-F: 9-12AM and 1-4PM.

Turnaround Time (TAT) varies based on the complexity and volume of projects, TAT for sequencing your own libraries is max 14 days from the date of sample drop off, NGS  for small projects that include libary prep and sequencing take up to 4 weeks, for large projects which include libary prep and sequencing of more than 50 samples expect a TAT of 6 weeks or more, TAT is calculated from the date of sample submission. Inquires "have you sequenced my samples/pool?" are not going to be answered.

Our services 

  • Project planning and study design   
  • Sample QC (Agilent Bioanalyzer, Tape Station, q-PCR)
  • Library prep: 
    • Whole Genome and Whole Exome  
    • Transcriptome sequencing (RNA-seq) 
    • Epigenome (Chip-seq, WGBS-seq, RRBS-seq) 
    • miRNA sequencing 
    • ATAC-seq
    • ctDNA sequencing (NGS provides an excellent technology that allows the detection of low levels of ctDNA in the blood in a highly sensitive and specific manner) 
  • Sequencing: NextSeq1000, NovaSeq6000, PromethION24.

 

To Reserve a qPCR instrument please select the "Schedule/Reserve your Instrument" tab, for everything else else please select the "Request Service" tab.

 

Please NoteExternal academic (Non CHOP/UPENN) researchers should add 10% to posted rate. External commercial customers should add 30% to posted rates. These charges will appear on the final invoice. 

 

Leadership

 Teodora Orendovici | Technical Director

Location and hours of operation

Hours Location

 

Monday - Friday

8:00 AM - 5:00 PM

 

A450 Colket Translational Research Building,

3501 Civic Center Boulevard, Philadelphia, PA, 19104

Links and Resources

  1. CHOP High Throughput Sequencing Core | CHOP Research Institute
  2. https://www.research.chop.edu/dbhi-bioinformatics

Contacts

Name Role Phone Email Location
Teodora Orendovici
Technical Director
 
814-574-1631
 
orendovict@chop.edu
 
CTRB A340B
 
Stephen Mahoney
Research Associate
 
215-896-6027
 
mahoney@chop.edu
 
CTRB A450D
 
CHOP HTS Core
General Inquiry
 
267-425-5866
 
HTSCore@chop.edu
 
CTRB A450D
 
Ashley Bushey
Research Technician
 
215-896-6027
 
busheya@chop.edu
 
CTRB A450D
 

Service list


Search available services: View: by category alphabetically
BeadBug Homogenizer (1)
Library Preparation Services (12)
Nanopore Sequencing – PromethION (5)
NextSeq Self Service (1)
NextSeq Services (7)
NovaSeq Self Service (2)
NovaSeq Services (12)
NovaSeq™ X Series Self Service (1)
NovaSeq™ X Series Services (1)
QC Bioanalyzer/TapeStation (10)
Sequencing run set up (1)
Surcharge (1)
qPCR (2)

Available Equipment and Resources

Realtime PCR, Self-serve (4)

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