CHOP High Throughput Sequencing Core

Overview of Services

The HT Sequencing Core is a full-service facility providing instrumentation, library preparation, and sequencing services to CHOP, UPenn, as well as to external investigators. Whether you want to sequence a library that you generated yourself, or assistance creating the library, we can help. Our goal is to enable high throughput sequencing as an in-house service to any laboratory and to make the HTS Core accessible to everyone interested in Next Generation Sequencing projects. 

The HT Sequencing Core has capabilities ranging from highly focused analysis of target DNA sequencing and miRNA to whole genome, epigenome, exome, and transcriptome sequencing. Services are available for a diversity of study designs; starting from a few samples to large projects involving hundreds of samples, we are working with investigators to provide optimized and customized protocols for each study. We understand that not all research is alike, we offer consulting services for custom experiments tailored to your research needs. We are here to listen and create solutions.


Our services 

  • Project planning and study design  
  • DNA/RNA isolation  
  • Sample QC (Agilent Bioanalyzer, q-PCR)
  • Library prep: 
    • Whole Genome and Whole Exome  
    • Other Targeted Sequencing (Cancer/Custom Panels) 
    • Transcriptome sequencing (RNA-seq) 
    • Epigenome (Chip-seq, BS-seq, RRBS-seq) 
    • miRNA sequencing 
    • Metagenomics 
    • ctDNA sequencing (NGS provides an excellent technology that allows the detection of low levels of ctDNA in the blood in a highly sensitive and specific manner) 
  • Sequencing: Miseq, NextSeq, and NovaSeq 


To Reserve an instrument please select the "Schedule/Reserve your Instrument" tab, for everything else else please select the "Request Service" tab.


Please NoteExternal academic (Non CHOP/UPENN) researchers should add 10% to posted rate. External commercial customers should add 30% to posted rates. These charges will appear on the final invoice. 



 Teodora Orendovici | Technical Director

Location and hours of operation

Hours Location


Monday - Friday

8:00 AM - 5:00 PM


A450 Colket Translational Research Building,

3501 Civic Center Boulevard, Philadelphia, PA, 19104

Links and Resources

  1. CHOP High Throughput Sequencing Core | CHOP Research Institute
  2. Bioinformatics resources  DBHi Bioinformatics | CHOP Research Institute


Name Role Phone Email Location
Teodora Orendovici
Technical Director
Colket Translational Research Center, Room A450D
Stephen Mahoney
Research Associate
Colket Translational Research Center, Room A450 D
General Inquiry

Service list

Search available services: View: by category alphabetically
Bioanalyzer (6)
Library Preparation Services (10)
MiSeq Sevices (6)
NextSeq Self Service (1)
NextSeq Services (3)
NovaSeq Self Service (1)
NovaSeq Services (10)
qPCR (1)

Available Equipment and Resources

Instruments (3)
Realtime PCR, Self-serve (4)