CHOP High Throughput Sequencing Core

Overview of Services

The HT Sequencing Core is a full-service NGS facility providing instrumentation, library preparation, and sequencing services to CHOP, UPenn, as well as to external investigators. Whether you want to sequence a library that you generated yourself, or assistance with library preparation, we can help. Our goal is to enable high throughput sequencing as an in-house service and to make the HTS Core accessible to everyone interested in Next Generation Sequencing projects. 

The HT Sequencing Core has capabilities ranging from highly focused analysis of target DNA sequencing and miRNA to whole genome, epigenome, exome, and transcriptome sequencing. Services are available for a diversity of study designs; starting with as few as 12 samples to large projects involving hundreds of samples, we are working with investigators to provide optimized protocols for each study. We understand that not all research is alike, we offer consulting services for custom experiments tailored to your research needs. We are here to listen and create solutions.

For any sequencing projects, except self-servce, please request a project ID. Samples can be submitted only after the requested service has been aproved in iLab and a project ID has been issued. For sample drop off please contact us through email, sample dropoff M-F: 9-12AM and 1-4PM.

Turnaround time (TAT) varies based on the complexity of the project, TAT for sequencing only is around 14 days, TAT for smaler projects that include libary prep and sequencing is 4 weeks, for larger projects which include libary prep and sequencing of more than 50 samples TAT is around 6 weeks or more, TAT is calculated from the date of sample submission. Inquires like "have you sequenced my samples/pool?" are not going to be answered.

Our services 

  • Project planning and study design  
  • DNA/RNA isolation  
  • Sample QC (Agilent Bioanalyzer, q-PCR)
  • Library prep: 
    • Whole Genome and Whole Exome  
    • Other Targeted Sequencing (Cancer/Custom Panels) 
    • Transcriptome sequencing (RNA-seq) 
    • Epigenome (Chip-seq, WGBS-seq, RRBS-seq) 
    • miRNA sequencing 
    • ATAC-seq
    • ctDNA sequencing (NGS provides an excellent technology that allows the detection of low levels of ctDNA in the blood in a highly sensitive and specific manner) 
  • Sequencing: NextSeq 1000, NovaSeq 6000, PromethION24.


To Reserve a qPCR instrument please select the "Schedule/Reserve your Instrument" tab, for everything else else please select the "Request Service" tab.


Please NoteExternal academic (Non CHOP/UPENN) researchers should add 10% to posted rate. External commercial customers should add 30% to posted rates. These charges will appear on the final invoice. 



 Teodora Orendovici | Technical Director

Location and hours of operation

Hours Location


Monday - Friday

8:00 AM - 5:00 PM


A450 Colket Translational Research Building,

3501 Civic Center Boulevard, Philadelphia, PA, 19104

Links and Resources

  1. CHOP High Throughput Sequencing Core | CHOP Research Institute


Name Role Phone Email Location
Teodora Orendovici
Technical Director
Stephen Mahoney
Research Associate
General Inquiry
Ashley Bushey
Research Technician

Service list

Search available services: View: by category alphabetically
Library Preparation Services (12)
NextSeq Self Service (1)
NextSeq Services (5)
NovaSeq Self Service (2)
NovaSeq Services (12)
QC Bioanalyzer/TapeStation (10)
Sequencing run set up (2)
qPCR (2)

Available Equipment and Resources

Realtime PCR, Self-serve (4)